The facility is equipped with a 4000 QTRAP LC/MS/MS system (AB Sciex), which is a hybrid triple quadrupole/linear ion trap mass spectrometer ideal for targeted small molecule quantitation. A Shimadzu UHPLC using 2 high pressure pumps is coupled to the QTRAP instrument for separation of complex samples. The following targeted methods are offered as full service: • Acidic Plant Hormone Analysis
We are using the 4000 QTRAP LC-MS/MS system for targeted analysis and quantitation of the following acidic plant hormones: jasmonic acid (JA), salicyclic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), jasmonoyl-isoleucine (JA-Ile), 12-oxo-phytodienoic acid (OPDA) and indole-3-acetyl-aspartic acid (IAA-Asp). The hormone extraction from plant tissues is performed by facility staff as it requires the spiking of deuterium-labeled internal standards at the beginning of the sample preparation. For more information, please see Plant Hormone
. • Amino Acid Analysis
We are using the AccQ•Tag system from Waters for derivatization followed by separation on a Waters Acquity UPLC system. It is ~9.5 min separation and we do either free amino acids (client extracted) or HCl hydrolysis (usually from tissue). We recommend samples be run in triplicate for appropriate statistical analysis. The chemistry is based on Waters' widely-used AccQ•Tag derivatizaton method. Primary and secondary amino acids react with a single reagent, AccQ•Fluor™, in a high-throughput batch process resulting in exceptionally stable derivatives. High resolution separations are achieved using pre-qualified AccQ•Tag Ultra UPLC columns and mobile phases. Derivatized amino acids are quantified at sub-picomole levels with single wavelength UV detection. For more information, please see AAA
. • Isoprenoid Biosynthesis Intermediates
We are using the 4000 QTRAP LC-MS/MS system for separation and quantification of 5 of the intermediates of the Non-mevolanate/methlerythritol/deoxyxyulose isoprenoid biosynthesis (MEP/DXP) pathway (DXP, MEP, CDP-ME, CDP-MEP* and cMEPP). The sample preparation is performed in house and samples are analyzed using a 20 minute run. For more information, please see this publication
We can extend our current methods to meet your needs or develop new methods for your particular projects. Please contact us for details.